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KMID : 0881720090240020124
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Journal of Food Hygiene and Safety
2009 Volume.24 No. 2 p.124 ~ p.127
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Evaluation of Estrogenic Activity of Pumpkin Seed Extract using Recombinant Yeast Assay
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Tahk Hong-Min
Lee Bog-Hieu
Rho Sook-Nyung
Kim Chun-Soo
Jung Ji-Youn
Choi Chang-Sun
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Abstract
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The aim of this study is to evaluate the estrogenic activity of Cucurbita pepo seed extract which includes and other phytosterols. Sample was extracted from Cucurbita pepo seed by supercritical carbondioxide method and resuspended with ethanol. Estrogenic activity was measured by recombinant yeast assay which detects estrogenic activity using recombinant yeast with high level of estrogenic receptor. However, estrogenic activity of pumpkin seed extract was not found in this study. Based on this data, pumpkin seed extract will not cause estrogenic disturbance.
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KEYWORD
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Cucurbita pepo, Phytoestrogen, Recombinant yeast assay
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